All most all the animal tissue contain DNA. We used mammalian blood for this practical. Only white blood cells in the mammalian blood contain nuclei. As RBC contain no nuclei, we have to remove the RBC first.
We destroyed RBC by a freez shock. For this we stored our blood samples at -20C over night. This freezing process destroys the RBC cell membrane.
Then before the practicle we thawed the blood samples at room temperature. After that 700ul of blood sample was transferred to an eppendorf tube.
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