Yesterday We finished Extracting DNA from Both Plant and Animal tissues. So now it's time to quantify our DNA samples and see how successful we were in extracting DNA.
We can quantify our DNA sample to measure the purity of the sample and to measure the amount of DNA present in our sample.
We added 50 - 100 ul of TE buffer to the dried DNA pallet.
TE buffer contains,
1M tris - 1ml
0.5M EDTA - 200ul
Distilled water up to - 100ml
Then the solution was homogenized by pipetting in and out about 30 times.
We can quantify our DNA sample to measure the purity of the sample and to measure the amount of DNA present in our sample.
We added 50 - 100 ul of TE buffer to the dried DNA pallet.
TE buffer contains,
1M tris - 1ml
0.5M EDTA - 200ul
Distilled water up to - 100ml
Then the solution was homogenized by pipetting in and out about 30 times.
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